How Do Proteins Precipitate?


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Arun Raj answered
The term precipitation is usually used for product recovery of biomolecules. This process is used to concentrate and extract the useful target product from contaminants. One example of the use of protein precipitation is discarding contaminants present in the blood, in the biotechnology industry. Protein precipitation is generally done by the addition of a salt in the form of an organic solvent. By protein precipitation, the pH solution is altered.

Salt induced precipitation is the most common form of salt-induced precipitation. When the concentration of salt is less in a solution; the solubility of protein increases marginally. When there is a high concentration of salt, the solubility of the protein falls sharply. This is called as "salting out" or protein precipitation.

Addition of an organic solvent can also decrease the solubility of the solution and cause protein precipitation. The third way of causing protein precipitation is changing the PH solution of the protein solution.

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